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sc 23949 rrid ab 628413  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology sc 23949 rrid ab 628413
    Sc 23949 Rrid Ab 628413, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3409 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B2+tubulin/%CE%B2+Tubulin+Antibody/pmc13657169-19-8-5
    Average 96 stars, based on 3409 article reviews
    sc 23949 rrid ab 628413 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Effects of Polygonum cognatum Meissn. through modulation of hyperglycaemia, oxidative stress via Nrf-2/HO-1, apoptosis, NF-κB/TLR-4 pathways.
    Article Snippet: 1 Department of Biochemistry, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey 2 Department of Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey Abstract The present study evaluated Polygonum cognatum extract (PCE) as a therapeutic agent for diabetes treatment.. The research used twenty-four Sprague–Dawley male rats which weighed between 250–300 g and were 90 days old.. The researchers distributed the 24 rats into four groups which included Control and Diabetes Mellitus (DM) and PCE and DM + PCE.

    Article Title: GABA Receptor Activation in Müller Glia as a Molecular Switch for Controlling VEGF-A in the Retina
    Article Snippet: .. Membranes were blocked with TBS-Tween 20 0.1% containing 5% skim milk for 1 hour at room temperature, then incubated overnight at 4 °C with primary antibodies against VEGF-A (1:100, sc-7269, Santa Cruz Biotechnology, USA) and β-tubulin (1:500, sc-5274, Santa Cruz Biotechnology, USA) in TBS-Tween 20 (0.1%) with 1% BSA. .. Subsequently, membranes were incubated with HRP-conjugated goat anti-mouse IgG (1:8000, Thermo Fisher, USA).

    Article Title: Targeting lipogenesis promotes the synergistic effect of the selective HDAC6 inhibitor ITF3756 with bortezomib in colon cancer cells
    Article Snippet: .. After blocking, the filter was incubated overnight at 4 °C with primary antibodies: anti-p53 (DO-1, 1:200, cat. No sc-126, Santa Cruz Biotechnology, CA, United States of America), anti-caspase 3/anti-cleaved caspase-3 (1:1,000, cat. No 9662S, Cell Signaling Technology, United States of America), anti-PPAR-γ (1:1,000, cat. Nosc-7273, Santa Cruz Biotechnology, CA, United States of America), anti-SREBP-1 (1:1,000, cat. No#bs-140R, BioSS, Dundee, United Kingdom), anti-PARP-1 (1:700, cat. No sc-8007, Santa Cruz Biotechnology, CA, United States of America), anti-α-acetylated-tubulin (1:1,000, cat. No T6793, Sigma Aldrich, Milan, Italy) and anti-γ-tubulin (1:10,000, cat. No T6557, Sigma Aldrich, Milan, Italy), lamin-B (1:1,000, cat. No365962, Santa Cruz Biotechnology, CA, United States of America) and β-tubulin (1:1,000, cat. No sc-55529, Santa Cruz Biotechnology). .. After washings in TBS-T, the membranes were incubated with appropriate HPR-conjugated secondary antibodies anti-Rabbit IgG (1:10,000, cat. No W4011, Promega Corporation, Madison, WI, United States of America) or anti-Mouse IgG (1:10,000, cat. No W4021, Promega Corporation, Madison, WI, United States of America) at room temperature for 1 h. The chemiluminescent signal was visualized by chemiluminescence solution (ECLTM Prime Western blotting Detection Reagents, cat. No RPN2232, AmershamTM, United Kingdom) and was detected using the ChemiDoc, XRS acquisition instrument (Bio-Rad, Hercules, CA, United States of America).

    Extraction:

    Article Title: Ginsenoside Rc inhibits diabetic cardiomyopathy by reducing lipotoxicity through the adiponectin and autophagy pathways.
    Article Snippet: Background: Diabetic cardiomyopathy (DCM), a consequential cardiovascular complication of diabetes mellitus, drives progressive myocardial fibrosis and cardiac dysfunction, culminating in heart failure (HF).. Ginsenoside Rc (Rc) has significant antidiabetic and cardiovascular protective properties, but its potential to improve DCM is

    Marker:

    Article Title: Inhibition of V-ATPase function drives apoptosis via GCN1/GCN2 kinase signaling
    Article Snippet: Immunoreactive bands were detected by incubating membranes with ECLTM Select Western Blotting Detection Reagent (RPN2235, CytivaTM) for 3 min before fluorescent signals were detected on a ChemidocMP (Bio-Rad). .. Antibodies were commercially obtained: PARP (#9542, Cell Signaling Technology), GAPDH (#2118, Cell Signaling Technology), β-Tubulin (sc-53140, Santa Cruz Biotechnology), phospho-H2A.X (07-164, Merck), anti-mouse (#7076, Cell Signaling Technology), anti-rat (#7077, Cell Signaling Technology), Caspase-3 (#9662,Cell Signaling Technology), XBP-1s (#12782, Cell Signaling Technology), ATF-6 (#65880, Cell Signaling Technology), α-Actinin (sc-17829, Santa Cruz), ATF-4 (#11815, Cell Signaling Technology), PERK (#3192, Cell Signaling Technology), eIF2α (#9722, Cell Signaling Technology), phospho-eIF2α (Ser51) (#9721, Cell Signaling Technology), GCN2 (#3302, Cell Signaling Technology), IRE1α (#3294,Cell Signaling Technology), Phospho-SAPK/JNK (Thr183/Tyr185)( #4668, Cell Signaling Technology), SAPK/JNK (#9252, Cell Signaling Technology), GAPDH (#2118, Cell Signaling Technology), CHOP (#2895, Cell Signaling Technology), BIM (#2933, Cell Signaling Technology), ZAKα (#BEYA301-993A-T, Szabo Scandic), PUMA (#4976 Cell Signaling Technology), NOXA (#14766, Cell Signaling Technology), BAX (#2772, Cell Signaling Technology), BAK(#3814, Cell Signaling Technology), MCL-1(#5452, Cell Signaling Technology); BCL-XL (#2762, Cell Signaling Technology); Protein markers used were either Broad Range Prestained Protein Marker (PL00002, Proteintech) or PageRuler TM Plus (26619, Thermo Scientific). ..

    other:

    Article Title: E-Cadherin Regulates HIF1-α In Vitro in Induced 3D Spheroid Models of Human Breast Cancer Through Both mTOR and Notch1 Signaling
    Article Snippet: Antibodies to housekeeping gene products included β-tubulin, #sc9104, Santa Cruz Biotechnology, and β-actin, #937215, R&D Systems, Minneapolis, MN, USA.

    Saline:

    Article Title: Lipoxin A4 improves myocardial ischemia/reperfusion injury through the Notch1-Nrf2 signaling pathway
    Article Snippet: Subsequently, polyacrylamide gel electrophoresis (PAGE) was conducted, and subsequent to this, the samples were transferred to membranes made of polyvinylidene fluoride membranes (Millipore, Eschborn, Germany). .. A 5% w/v BSA solution was added to phosphate-buffered saline (PBS) containing 0.1% Tween 20 and sealed at ordinary temperatures for 1 h. Primary antibodies, including β-tubulin and β-actin from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and Anti-Notch1, hairy and enhancer of split 1 (Hes1), and Nrf2 antibodies from Abcam (Cambridge, MA, USA), were diluted and added according to the instructions. .. Suitable secondary antibodies were diluted (1:1,000–1:2,000 v/v) in PBS with 0.1% Tween 20 at ordinary temperatures and incubated for 1 h. Signal detection was performed using the ECL reagent kit (Thermo Scientific Pierce, Rockford, IL, USA).

    Western Blot:

    Article Title: Epigenetic regulation of ACSL4 via H2A monoubiquitylation connects lipid metabolism to BAP1-mediated ferroptosis.
    Article Snippet: .. The primary antibodies used for Western blotting assays were as follows: β-Tubulin (66240-1-Ig), Vinculin (66305-1-Ig), HIF1α (20960-1-AP), STING (19851-1-AP), GAPDH (HRP-60004) were bought from Proteintech (Wuhan, China); ACSL4 (sc-271800) was purchased from Santa Cruz Biotechnology (Dallas, Texas, USA); β-actin (AC004) was obtained from ABclonal Technology (Wuhan, China); BAP1 (#13271S), SLC7A11 (#12691), H2Aub (#8240S), H2A (#12349S), ASXL1 (#52519s), ASXL2 (#71257s), OGT (#5368), Flag-tag (#14793), Myc-tag (#2276) were obtained from Cell Signaling Technology (Danvers, Massachusetts, USA); c-Myc (#0912-2) was obtained from HUABIO (Hangzhou, China); FOXK1 (ab18196) and KDM1B (ab193080) were obtained from Abcam (Cambridge Biomedical Campus, Cambridge, Cell Death & Differentiation Fig. 6 ASXLs are required for the regulation of gene expression and ferroptosis by BAP1. ..

    Cell-Signaling:

    Article Title: Epigenetic regulation of ACSL4 via H2A monoubiquitylation connects lipid metabolism to BAP1-mediated ferroptosis.
    Article Snippet: .. The primary antibodies used for Western blotting assays were as follows: β-Tubulin (66240-1-Ig), Vinculin (66305-1-Ig), HIF1α (20960-1-AP), STING (19851-1-AP), GAPDH (HRP-60004) were bought from Proteintech (Wuhan, China); ACSL4 (sc-271800) was purchased from Santa Cruz Biotechnology (Dallas, Texas, USA); β-actin (AC004) was obtained from ABclonal Technology (Wuhan, China); BAP1 (#13271S), SLC7A11 (#12691), H2Aub (#8240S), H2A (#12349S), ASXL1 (#52519s), ASXL2 (#71257s), OGT (#5368), Flag-tag (#14793), Myc-tag (#2276) were obtained from Cell Signaling Technology (Danvers, Massachusetts, USA); c-Myc (#0912-2) was obtained from HUABIO (Hangzhou, China); FOXK1 (ab18196) and KDM1B (ab193080) were obtained from Abcam (Cambridge Biomedical Campus, Cambridge, Cell Death & Differentiation Fig. 6 ASXLs are required for the regulation of gene expression and ferroptosis by BAP1. ..

    Gene Expression:

    Article Title: Epigenetic regulation of ACSL4 via H2A monoubiquitylation connects lipid metabolism to BAP1-mediated ferroptosis.
    Article Snippet: .. The primary antibodies used for Western blotting assays were as follows: β-Tubulin (66240-1-Ig), Vinculin (66305-1-Ig), HIF1α (20960-1-AP), STING (19851-1-AP), GAPDH (HRP-60004) were bought from Proteintech (Wuhan, China); ACSL4 (sc-271800) was purchased from Santa Cruz Biotechnology (Dallas, Texas, USA); β-actin (AC004) was obtained from ABclonal Technology (Wuhan, China); BAP1 (#13271S), SLC7A11 (#12691), H2Aub (#8240S), H2A (#12349S), ASXL1 (#52519s), ASXL2 (#71257s), OGT (#5368), Flag-tag (#14793), Myc-tag (#2276) were obtained from Cell Signaling Technology (Danvers, Massachusetts, USA); c-Myc (#0912-2) was obtained from HUABIO (Hangzhou, China); FOXK1 (ab18196) and KDM1B (ab193080) were obtained from Abcam (Cambridge Biomedical Campus, Cambridge, Cell Death & Differentiation Fig. 6 ASXLs are required for the regulation of gene expression and ferroptosis by BAP1. ..

    Blocking Assay:

    Article Title: Targeting lipogenesis promotes the synergistic effect of the selective HDAC6 inhibitor ITF3756 with bortezomib in colon cancer cells
    Article Snippet: .. After blocking, the filter was incubated overnight at 4 °C with primary antibodies: anti-p53 (DO-1, 1:200, cat. No sc-126, Santa Cruz Biotechnology, CA, United States of America), anti-caspase 3/anti-cleaved caspase-3 (1:1,000, cat. No 9662S, Cell Signaling Technology, United States of America), anti-PPAR-γ (1:1,000, cat. Nosc-7273, Santa Cruz Biotechnology, CA, United States of America), anti-SREBP-1 (1:1,000, cat. No#bs-140R, BioSS, Dundee, United Kingdom), anti-PARP-1 (1:700, cat. No sc-8007, Santa Cruz Biotechnology, CA, United States of America), anti-α-acetylated-tubulin (1:1,000, cat. No T6793, Sigma Aldrich, Milan, Italy) and anti-γ-tubulin (1:10,000, cat. No T6557, Sigma Aldrich, Milan, Italy), lamin-B (1:1,000, cat. No365962, Santa Cruz Biotechnology, CA, United States of America) and β-tubulin (1:1,000, cat. No sc-55529, Santa Cruz Biotechnology). .. After washings in TBS-T, the membranes were incubated with appropriate HPR-conjugated secondary antibodies anti-Rabbit IgG (1:10,000, cat. No W4011, Promega Corporation, Madison, WI, United States of America) or anti-Mouse IgG (1:10,000, cat. No W4021, Promega Corporation, Madison, WI, United States of America) at room temperature for 1 h. The chemiluminescent signal was visualized by chemiluminescence solution (ECLTM Prime Western blotting Detection Reagents, cat. No RPN2232, AmershamTM, United Kingdom) and was detected using the ChemiDoc, XRS acquisition instrument (Bio-Rad, Hercules, CA, United States of America).



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    Image Search Results


    IL‐35 Subunit EBI3 is Downregulated in both Obese Mouse Aortas and PA‐Treated HUVECs, and Restored by Exogenous IL‐35. (A, B) Immunofluorescence of EBI3 (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (C, D) Immunofluorescence of IL‐12A (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (E) Serum IL‐35 concentrations, n = 8. (F, H) Representative Western blot bands of EBI3 and IL‐12A in aortic tissues (F) and densitometric analysis (H, n = 4). (G, I) Representative Western blot bands of EBI3 and IL‐12A in HUVECs (G) and densitometric analysis (I, n = 4). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 Subunit EBI3 is Downregulated in both Obese Mouse Aortas and PA‐Treated HUVECs, and Restored by Exogenous IL‐35. (A, B) Immunofluorescence of EBI3 (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (C, D) Immunofluorescence of IL‐12A (red) with CD31 (green) and DAPI (blue) in mouse aortas, with semi‐quantification of fluorescence intensity, n = 3 (scale bar = 50 µM). (E) Serum IL‐35 concentrations, n = 8. (F, H) Representative Western blot bands of EBI3 and IL‐12A in aortic tissues (F) and densitometric analysis (H, n = 4). (G, I) Representative Western blot bands of EBI3 and IL‐12A in HUVECs (G) and densitometric analysis (I, n = 4). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary antibodies for immunofluorescence and Western blotting: EBI3 (12371‐1‐AP), IL‐12A (85754‐2‐RR), platelet endothelial cell adhesion molecule‐1 (PECAM‐1/CD31) (11265‐1‐AP), eNOS (27120‐1‐AP), and β‐tubulin (66240‐1‐Ig) from Proteintech (USA); p‐eNOS (Ser1177, #9571), p‐cRAF (#9427), p‐MEK1/2 (#9154), and p‐STAT3 (#9145) from Cell Signaling Technology (USA); cRAF (A19638), MEK1/2 (A4868), p‐ERK1/2 (AP0974), ERK1/2 (A4782), and STAT3 (A1192) from ABclonal Technology (China).

    Techniques: Immunofluorescence, Fluorescence, Western Blot, Analysis

    IL‐35 Restores eNOS Ser1177 phosphorylation and nitric oxide production to enhance endothelium‐dependent relaxation in obesity. (A) Concentration‐response curves of endothelium‐dependent relaxation to ACh and comparison of maximal relaxation (Emax) in mouse aortic rings, n = 4. (B) Concentration‐response curves of endothelium‐independent relaxation to SNP and comparison of Emax, n = 4. (C, D) Western blot bands of p‐eNOS (Ser1177) and total eNOS in mouse aortic tissues (C) and HUVECs (D). (E, F) Densitometric analysis of total eNOS and p‐eNOS (Ser1177)/total eNOS in aortic tissues (E, n = 4) and HUVECs (F, n = 4). (G) NO fluorescence intensity in HUVECs, scale bar = 50 μM. (H) Semi‐quantification of NO fluorescence intensity in HUVECs, n = 3. Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 Restores eNOS Ser1177 phosphorylation and nitric oxide production to enhance endothelium‐dependent relaxation in obesity. (A) Concentration‐response curves of endothelium‐dependent relaxation to ACh and comparison of maximal relaxation (Emax) in mouse aortic rings, n = 4. (B) Concentration‐response curves of endothelium‐independent relaxation to SNP and comparison of Emax, n = 4. (C, D) Western blot bands of p‐eNOS (Ser1177) and total eNOS in mouse aortic tissues (C) and HUVECs (D). (E, F) Densitometric analysis of total eNOS and p‐eNOS (Ser1177)/total eNOS in aortic tissues (E, n = 4) and HUVECs (F, n = 4). (G) NO fluorescence intensity in HUVECs, scale bar = 50 μM. (H) Semi‐quantification of NO fluorescence intensity in HUVECs, n = 3. Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary antibodies for immunofluorescence and Western blotting: EBI3 (12371‐1‐AP), IL‐12A (85754‐2‐RR), platelet endothelial cell adhesion molecule‐1 (PECAM‐1/CD31) (11265‐1‐AP), eNOS (27120‐1‐AP), and β‐tubulin (66240‐1‐Ig) from Proteintech (USA); p‐eNOS (Ser1177, #9571), p‐cRAF (#9427), p‐MEK1/2 (#9154), and p‐STAT3 (#9145) from Cell Signaling Technology (USA); cRAF (A19638), MEK1/2 (A4868), p‐ERK1/2 (AP0974), ERK1/2 (A4782), and STAT3 (A1192) from ABclonal Technology (China).

    Techniques: Phospho-proteomics, Concentration Assay, Comparison, Western Blot, Analysis, Fluorescence

    IL‐35 induces the MAPK/ERK signaling pathway, thereby enhancing eNOS Ser1177 phosphorylation and NO production in endothelial cells. (A) Volcano plot of eNOS‐interacting proteins in HUVECs (PA vs. PA + IL‐35) by IP‐mass spectrometry. Red dots: upregulated in PA + IL‐35 group (fold change > 1.5, p < 0.05); MAPK3 (ERK1) is highlighted. (B) STRING‐based PPI network of top 20 binding proteins. ERK1 is the only direct interactor with eNOS. (C) Western blot bands of p‐cRAF, p‐MEK, p‐ERK, p‐eNOS (Ser1177), and total proteins in HUVECs. (D–G) Densitometric analysis of p‐cRAF, p‐MEK, p‐ERK, and p‐eNOS (Ser1177) normalized to total proteins, n = 3. (H, I) NO fluorescence intensity in HUVECs (H, scale bar = 50 μM) with semi‐quantification (I, n = 3). Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 induces the MAPK/ERK signaling pathway, thereby enhancing eNOS Ser1177 phosphorylation and NO production in endothelial cells. (A) Volcano plot of eNOS‐interacting proteins in HUVECs (PA vs. PA + IL‐35) by IP‐mass spectrometry. Red dots: upregulated in PA + IL‐35 group (fold change > 1.5, p < 0.05); MAPK3 (ERK1) is highlighted. (B) STRING‐based PPI network of top 20 binding proteins. ERK1 is the only direct interactor with eNOS. (C) Western blot bands of p‐cRAF, p‐MEK, p‐ERK, p‐eNOS (Ser1177), and total proteins in HUVECs. (D–G) Densitometric analysis of p‐cRAF, p‐MEK, p‐ERK, and p‐eNOS (Ser1177) normalized to total proteins, n = 3. (H, I) NO fluorescence intensity in HUVECs (H, scale bar = 50 μM) with semi‐quantification (I, n = 3). Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Primary antibodies for immunofluorescence and Western blotting: EBI3 (12371‐1‐AP), IL‐12A (85754‐2‐RR), platelet endothelial cell adhesion molecule‐1 (PECAM‐1/CD31) (11265‐1‐AP), eNOS (27120‐1‐AP), and β‐tubulin (66240‐1‐Ig) from Proteintech (USA); p‐eNOS (Ser1177, #9571), p‐cRAF (#9427), p‐MEK1/2 (#9154), and p‐STAT3 (#9145) from Cell Signaling Technology (USA); cRAF (A19638), MEK1/2 (A4868), p‐ERK1/2 (AP0974), ERK1/2 (A4782), and STAT3 (A1192) from ABclonal Technology (China).

    Techniques: Phospho-proteomics, Mass Spectrometry, Binding Assay, Western Blot, Analysis, Fluorescence

    IL‐35 activates the STAT3‐MAPK/ERK‐eNOS signaling axis to promote eNOS phosphorylation and NO production in endothelial cells. (A) Western blot bands of p‐STAT3, p‐eNOS (Ser1177), p‐cRAF, p‐MEK, p‐ERK, and corresponding total proteins in HUVECs. (B–I) Densitometric analysis of p‐STAT3, STAT3, p‐eNOS (Ser1177), eNOS, p‐cRAF, p‐MEK, and p‐ERK normalized to total proteins or β‐tubulin, n = 3. (J–K) NO fluorescence intensity in HUVECs (J, scale bar = 50 μM) with semi‐quantification (K, n = 3). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Immunity, Inflammation and Disease

    Article Title: Interleukin‐35 Ameliorates Obesity‐Associated Impairment of Endothelium‐Dependent Vasodilation via the STAT3‐MAPK/ERK‐eNOS Signaling Axis

    doi: 10.1002/iid3.70564

    Figure Lengend Snippet: IL‐35 activates the STAT3‐MAPK/ERK‐eNOS signaling axis to promote eNOS phosphorylation and NO production in endothelial cells. (A) Western blot bands of p‐STAT3, p‐eNOS (Ser1177), p‐cRAF, p‐MEK, p‐ERK, and corresponding total proteins in HUVECs. (B–I) Densitometric analysis of p‐STAT3, STAT3, p‐eNOS (Ser1177), eNOS, p‐cRAF, p‐MEK, and p‐ERK normalized to total proteins or β‐tubulin, n = 3. (J–K) NO fluorescence intensity in HUVECs (J, scale bar = 50 μM) with semi‐quantification (K, n = 3). Data are mean ± SD. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Primary antibodies for immunofluorescence and Western blotting: EBI3 (12371‐1‐AP), IL‐12A (85754‐2‐RR), platelet endothelial cell adhesion molecule‐1 (PECAM‐1/CD31) (11265‐1‐AP), eNOS (27120‐1‐AP), and β‐tubulin (66240‐1‐Ig) from Proteintech (USA); p‐eNOS (Ser1177, #9571), p‐cRAF (#9427), p‐MEK1/2 (#9154), and p‐STAT3 (#9145) from Cell Signaling Technology (USA); cRAF (A19638), MEK1/2 (A4868), p‐ERK1/2 (AP0974), ERK1/2 (A4782), and STAT3 (A1192) from ABclonal Technology (China).

    Techniques: Phospho-proteomics, Western Blot, Analysis, Fluorescence

    Journal: iScience

    Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system

    doi: 10.1016/j.isci.2026.117149

    Figure Lengend Snippet:

    Article Snippet: rabbit anti-β-tubulin , Proteintech, Wuhan, China , Cat#10094-1-AP;RRID:AB_2210695.

    Techniques: Transfection, Cloning, Imaging, Bicinchoninic Acid Protein Assay, Western Blot, Plasmid Preparation, Recombinant, Software